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| 1. |  | ROCHA, F. S. da; JESUS, V. .L. T. de; TORRES, H. M.; GOMES, M. J. P.; FIGUEIREDO, M. J. de; NASCIMENTO, E. R. do; FERREIRA, T.; AQUINO, M. H. C. de Investigação de Campylobacter fetus e Tritrichomonas foetus na mucosa prepucial de touros da região do Médio Paraíba, RJ. Ciência Rural, Santa Maria, v.39, n.5, p.1586-1589, ago. 2009.| Biblioteca(s): Epagri-Sede. |
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Registro Completo
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Biblioteca(s): |
Epagri-Sede. |
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Data corrente: |
14/03/2017 |
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Data da última atualização: |
14/03/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
Internacional - B |
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Autoria: |
GARCIA, S.; YASUI, G. S.; BERNARDES-JUNIOR, J.; AMARAL JÚNIOR, H.; SILVA, B. C.; ZANIBONI-FILHO, E. |
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Título: |
Induction of triploidy in Rhamdia quelen (Siluriformes, Heptapteridae) by double-temperature shock. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Latin American Journal of Aquatic Research, Chile, v. 45, n. 1, p. 209-212, 2017. |
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Idioma: |
Inglês |
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Conteúdo: |
This study evaluated sequential double-temperature shock (heat and cold) to induce triploidy in Rhamdia quelen. Fertilized eggs were heat shocked at 37, 39 and 41°C at 2 min post-fertilization and then cold shocked at 1°C for 20 min. Intact eggs were used as controls. Egg viability in control groups was higher (65.5 ± 37.0%) than that at 37°C (58.2 ± 37.7%), 39°C (1.8 ± 0.3%) and 41ºC (0%). The survival of control hatchery (64.6 ± 36.8%) was higher compared to treatments at 37ºC (24.4 ± 15.5%) and 39ºC (0.6 ± 0.07%). Triploid percentages were 98.5% at 37°C and 100% at 39°C. Therefore, while double-temperature shock induced 100% triploidy in R. quelen, it also significantly decreased embryo survival and increased the deformity larvae. |
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Palavras-Chave: |
flow cytometry; polyploidy; Rhamdia quelen; silver catfish; temperature shock. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 01501naa a2200241 a 4500
001 1126039
005 2017-03-14
008 2017 bl uuuu u00u1 u #d
100 1 $aGARCIA, S.
245 $aInduction of triploidy in Rhamdia quelen (Siluriformes, Heptapteridae) by double-temperature shock.$h[electronic resource]
260 $c2017
520 $aThis study evaluated sequential double-temperature shock (heat and cold) to induce triploidy in Rhamdia quelen. Fertilized eggs were heat shocked at 37, 39 and 41°C at 2 min post-fertilization and then cold shocked at 1°C for 20 min. Intact eggs were used as controls. Egg viability in control groups was higher (65.5 ± 37.0%) than that at 37°C (58.2 ± 37.7%), 39°C (1.8 ± 0.3%) and 41ºC (0%). The survival of control hatchery (64.6 ± 36.8%) was higher compared to treatments at 37ºC (24.4 ± 15.5%) and 39ºC (0.6 ± 0.07%). Triploid percentages were 98.5% at 37°C and 100% at 39°C. Therefore, while double-temperature shock induced 100% triploidy in R. quelen, it also significantly decreased embryo survival and increased the deformity larvae.
653 $aflow cytometry
653 $apolyploidy
653 $aRhamdia quelen
653 $asilver catfish
653 $atemperature shock
700 1 $aYASUI, G. S.
700 1 $aBERNARDES-JUNIOR, J.
700 1 $aAMARAL JÚNIOR, H.
700 1 $aSILVA, B. C.
700 1 $aZANIBONI-FILHO, E.
773 $tLatin American Journal of Aquatic Research, Chile$gv. 45, n. 1, p. 209-212, 2017.
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